NAME Cell Culture Platform

DESCRIPTION

The Cell Culture Platform (PCC) includes three cell culture rooms: two rooms are dedicated to the cultivation of primary and established cell lines, and the third room is used to infect cells with viruses under level 2 biosafety conditions. The platform is currently active in three areas: (i) Research: the PCC is responsible for the correct operation and preventive maintenance of cultivation equipment, cleaning surveillance, waste management, biosecurity, microbiological controls and mycoplasmas. The services offered to external researchers include all the usual assignments of tasks related to cell culture. (ii) Technological development: the platform provides a service to test new culture-related products before commercialization (such as cell culture plates, factors and antibodies) and other advanced research tools. (iii) Cell-based therapies: industrial developments of advanced therapy drugs (cells, genes and tissues) are increasingly reaching hospitals in the form of clinical trials led by the biotechnology and pharmaceutical industry. However, a major bottleneck for allogeneic cell-based products, which are generally shipped frozen, is handling of the cell product prior to infusion. Our hospital has quickly become one of the few national referral sites because we have the relevant experience and infrastructure to support cell-based assays.

FIELDS OF APPLICATION

In-Vitro diagnostics

Laboratory equipment

MOST OUTSTANDING EQUIPMENT AND COMPONENTS

  • Fluorescence microscope Nikon ECLIPSE 80i

    DAPI, FITC, Texas Red, Triple and far red filters

  • Guaca Easycyte 8HT flow cytometer

    Blue (488 nm) and red (640 nm) laser (up to 6 fluorescence colour visualization and 2 light scatters)

  • SeaHorse XFe96 Analyzer

    SeaHorse XFe96 Analyzer

SERVICES OFFERED BY THE ASSET

Cell line authentication

Cell line authentication

Cell line culture maintenance

Under sterilized conditions

Cell metabolism analysis

Through SeaHorse XFe96 analyser, the platform offers mitochondrial function measurements, glycolytic function and fatty-acid oxidation

Cell proliferation and viability assays

Cell proliferation and viability assays

Cell type isolation

Human and animal model tissue processing for primary cell culture isolation.

Flow Cytometry Analysis

Flow Cytometry Analysis

Immortalized cell lines culture

Maintenance, expansion and cryopreservation of commercial cell lines.

Immunofluorescence

For the observation and analysis of samples of a very diverse nature (cells, tissues, etc.)

Myoplasm testing

Myoplasm testing

Preclinical novel compound testing in cell culture

Such as cell culture plates, factors and antibodies and other advanced research tools.

Transfection assays

Transfection assays

ENTITY MANAGING THE ASSET

ENTITY MANAGING THE ASSET
Biodonostia Health Research Institute
Contact person:
IIS Biodonostia
amaia.delvillar@biodonostia.org