NAME Genomics platform
The Genomics Platform offers advice for, and interaction with, research groups to help them implement the most appropriate genomic and transcriptomic techniques for the biological problems being studied and to help them design and/or refine new experimental approaches. For that purpose, the platform has a qualified staff, the latest techniques and equipment for sequencing, genotyping and analyzing gene expression and a lab which was designed following recommendations based on good laboratory practices.
FIELDS OF APPLICATION
Biomedical consumables
In-Vitro diagnostics
Laboratory equipment
MOST OUTSTANDING EQUIPMENT AND COMPONENTS
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7900HT real time PCR system
7900HT real time PCR system
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ABI3130xl sequencer
ABI3130xl sequencer
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Bio-Rad Droplet Digital PCR (ddPCR)
Bio-Rad Droplet Digital PCR (ddPCR)
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GeneChip Affymetrix genetic analysis system
GeneChip Affymetrix genetic analysis system
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PGM Ion Torrent - Next Generation Sequencing
PGM Ion Torrent - Next Generation Sequencing
SERVICES OFFERED BY THE ASSET
Bidimensional gels scanning-DIGE
Bidimensional gels scanning-DIGE
DNA and RNA Quality analysis and quantification
DNA and RNA Quality analysis and quantification
Human cell lines authentication
Identification and authentication of human cell lines and intra-species cross contamination with detection with GenePrint® 10 System de Promega
MAGPIX (Luminex)
Protein and nucleic acid qualitative and quantitative analysis
Massive sequencing (PGM – Ion Torrent)
Complete sequencing of reduced and viral genomes, mitochondrial sequencing, sequencing and quantification of microRNAs, sequencing of kits on demand with genes of interest
Microarray expression analysis
GeneChip Affymetrix Platform
PCR digital (Droplet Digital polymerase chain reaction – ddPCR)
Absolute quantification without a standard curve, genomic alterations such as copy number variation (CNV), rare sequences, gene expression and microRNA analysis, massive sequencing -NGS- (quantification of libraries; validation of results) and single cell analysis.
Real time qPCR
With specific primers to determine number of copies or relative quantity of a specific DNA or RNA sequence.
Sanger sequencing services
Sanger sequencing services (1100-1300 pb fragments), Fragment analysis
ENTITY MANAGING THE ASSET
Contact person:
IIS Biodonostia
amaia.delvillar@biodonostia.org