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NAME

Cell Culture Platform

DESCRIPTION

The Cell Culture Platform (PCC) includes three cell culture rooms: two rooms are dedicated to the cultivation of primary and established cell lines, and the third room is used to infect cells with viruses under level 2 biosafety conditions. The platform is currently active in three areas: (i) Research: the PCC is responsible for the correct operation and preventive maintenance of cultivation equipment, cleaning surveillance, waste management, biosecurity, microbiological controls and mycoplasmas. The services offered to external researchers include all the usual assignments of tasks related to cell culture. (ii) Technological development: the platform provides a service to test new culture-related products before commercialization (such as cell culture plates, factors and antibodies) and other advanced research tools. (iii) Cell-based therapies: industrial developments of advanced therapy drugs (cells, genes and tissues) are increasingly reaching hospitals in the form of clinical trials led by the biotechnology and pharmaceutical industry. However, a major bottleneck for allogeneic cell-based products, which are generally shipped frozen, is handling of the cell product prior to infusion. Our hospital has quickly become one of the few national referral sites because we have the relevant experience and infrastructure to support cell-based assays.

MOST OUTSTANDING EQUIPMENT AND COMPONENTS

Fluorescence microscope Nikon ECLIPSE 80i +

DAPI, FITC, Texas Red, Triple and far red filters

Guaca Easycyte 8HT flow cytometer +

Blue (488 nm) and red (640 nm) laser (up to 6 fluorescence colour visualization and 2 light scatters)

SeaHorse XFe96 Analyzer +

SeaHorse XFe96 Analyzer

SERVICES OFFERED BY THE ASSET

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1055

Cell line authentication

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1051

Cell line culture maintenance

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1048

Cell metabolism analysis

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1052

Cell proliferation and viability assays

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1049

Cell type isolation

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1057

Flow Cytometry Analysis

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1050

Immortalized cell lines culture

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1056

Immunofluorescence

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1054

Myoplasm testing

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1058

Preclinical novel compound testing in cell culture

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1053

Transfection assays

ENTITY MANAGING THE ASSET

Biodonostia Health Research Institute

,

Contact person:

IIS Biodonostia

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